Phospho-Caspase-9 (Thr125) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB5767
- Bilder (7)
| Artikelname: | Phospho-Caspase-9 (Thr125) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB5767 |
| Hersteller Artikelnummer: | orb5767 |
| Alternativnummer: | BYT-ORB5767-50,BYT-ORB5767-100,BYT-ORB5767-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human Caspase-9 around the phosphorylation site of Thr125 PE(p-T)PR |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CASP9 | Caspase-9 (p-T125), p-Caspase-9, phospho-Caspase-9, APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56, CASP-9, Caspase-9, Casp-9-CTD, Casp9_v1, CASP9_HUMAN, CASP9, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3 (APAF-3), ICE-like apoptotic protease 6 (ICE-LAP6), 3.4.22.62, Q4FJK5_MOUSE, Apoptotic protease-activating factor 3, ICE-like apoptotic protease 6, Q920G4_RAT, |
| Phospho-Caspase-9 (Thr125) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 35 kDa |
| UniProt: | P55211 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | CASP9 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2µg /test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Caspase-9 (Thr125) antibody (orb5767), dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Caspase-9 (Thr125)) Polyclonal Antibody, Unconjugated (orb5767) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-caspase-9 (Thr125)) Polyclonal Antibody, Unconjugated (orb5767) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (P-caspase-9 (Thr125)) Polyclonal Antibody, Unconjugated (orb5767) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Sample: Brain (Rat) Lysate at 40 ug, Primary: Anti-p-Caspase-9 (Thr125) (orb5767) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 35kD, Observed band size: 35kD. |
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Sample: H9C2 (Rat) cell Lysate at 40 ug, Primary: Anti-p-Caspase-9 (Thr125) (orb5767) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 35kD, Observed band size: 35kD. |
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Sample: Heart (Rat) Lysate at 40 ug, Heart (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Caspase-9 (Thr125) (orb5767) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46-51/35/37 kD, Observed band size: 51/37/35 kD. |
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