NOTCH1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB577050
Artikelname: NOTCH1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB577050
Hersteller Artikelnummer: orb577050
Alternativnummer: BYT-ORB577050-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NOTCH1
Konjugation: Unconjugated
Alternative Synonym: hN1, AOS5, TAN1, AOVD1
Rabbit polyclonal antibody to NOTCH1
Klonalität: Polyclonal
Konzentration: 0.5 mg/ml
Molekulargewicht: 273 kDa
NCBI: 060087
UniProt: P46531
Puffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Formulierung: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequenz: Synthetic peptide located within the following region: EHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK
Target-Kategorie: NOTCH1
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Full length NOTCH1 (~273 kDa) is not shown in this experiment, cleavage products of NOTCH1 are detected including the NOTCH Intracellular Domain at 88 kDa.
Sample Tissue: Human HepG2 Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Tissue: Human HT1080 Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Tissue: Human PANC1 Whole Cell, Antibody Dilution: 1 ug/ml.
Sample Tissue: Human U937 Whole Cell, Antibody Dilution: 1 ug/ml.
Rabbit Anti-NOTCH1 Antibody, Catalog Number: orb577050, Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue, Observed Staining: Plasma membrane, Primary Antibody Concentration: 1:100, Other Working Concentrations: 1:600, Secondary Antibody: Donkey anti-Rabbit-Cy3, Secondary Antibody Concentration: 1:200, Magnification: 20X, Exposure Time: 0.5-2.0 sec.
WB Suggested Anti-NOTCH1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 293T cell lysate.