A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Konjugation:
Unconjugated
EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas, in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Application Verdünnung:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Anwendungsbeschreibung:
Application Notes: Titering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human MCF7 cell lysate with recombinant EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Western blot testing of human lung lysate with EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.
IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
