Phospho-ATM (Ser1981) Recombinant Rabbit Monoclonal Antibody, Clone: [3F10], Unconjugated
Artikelnummer:
BYT-ORB608078
- Bilder (8)
| Artikelname: | Phospho-ATM (Ser1981) Recombinant Rabbit Monoclonal Antibody, Clone: [3F10], Unconjugated |
| Artikelnummer: | BYT-ORB608078 |
| Hersteller Artikelnummer: | orb608078 |
| Alternativnummer: | BYT-ORB608078-100,BYT-ORB608078-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | A synthesized peptide derived from human ATM around the phosphorylation site of S1981 EG-pS-QS |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ATM (p-S1981), p-ATM, phospho-ATM, AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1, C030026E19Rik, ATM_HUMAN, ATM, Ataxia telangiectasia mutated (A-T mutated), 2.7.11.1, ATM_MOUSE, Ataxia telangiectasia mutated homolog (A-T mutated homolog), THIL_RAT, Acat1, Acetoacetyl-CoA thiolase, 2.3.1.9, |
| Phospho-ATM (Ser1981) Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [3F10] |
| Molekulargewicht: | 370 kDa |
| UniProt: | Q13315 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | ATM |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-ATM (Ser1981)) Monoclonal Antibody, Unconjugated (orb608078) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: HepG2 cell lysates, Primary: Anti-phospho-ATM (Ser1981) (orb608078) at 1:1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:20000 dilution, Predicted band size: 370 kD, Observed band size: 350 kD. |
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Sample: Lane 1: Normal Human HeLa cell lysates, Lane 2: HeLa cell treated with 1 µM camptothecin (CPT) for 1 hour, Primary: Anti-phospho-ATM (Ser1981) (orb608078) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 370 kDa, Observed band size: 65 kDa. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (phospho S1981)) monoclonal Antibody, Unconjugated (orb608078) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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