Glucagon Receptor Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB6093
Artikelname: Glucagon Receptor Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB6093
Hersteller Artikelnummer: orb6093
Alternativnummer: BYT-ORB6093-50,BYT-ORB6093-100,BYT-ORB6093-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC, IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Glucagon Receptor (11-110/477aa)
Konjugation: Unconjugated
Alternative Synonym: GGR, GL-R, MVAH, GR, GLR_HUMAN, GCGR, GLR_MOUSE, GLR_RAT,
Glucagon Receptor Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 51 kDa
UniProt: P47871
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: GCGR
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Immunohistochemical analysis of paraffin-embedded rat myocardium using Glucagon Receptor antibody .
Blank control (blue): Molt-4 Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (orb6093/AF647), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF647 (orange), used under the same conditions.
Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Glucagon Receptor antibody (orb6093), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (human stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (orb6093) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated (orb6093) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebellum (Mouse) Lysate at 40 ug, Primary: Anti-Glucagon Receptor (orb6093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD.
Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-Glucagon Receptor (orb6093) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51kD, Observed band size: 51kD.



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