NRF1 Recombinant Rabbit Monoclonal Antibody, Clone: [6G5], Unconjugated
Artikelnummer:
BYT-ORB612207
- Bilder (8)
| Artikelname: | NRF1 Recombinant Rabbit Monoclonal Antibody, Clone: [6G5], Unconjugated |
| Artikelnummer: | BYT-ORB612207 |
| Hersteller Artikelnummer: | orb612207 |
| Alternativnummer: | BYT-ORB612207-50,BYT-ORB612207-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human NRF1 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ALPHA-PAL, NRF1_HUMAN, NRF1, NRF-1, Alpha palindromic-binding protein (Alpha-pal), |
| NRF1 Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [6G5] |
| Molekulargewicht: | 55 kDa |
| UniProt: | Q16656 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | NRF1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100 |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (orb612207) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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MCF-7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (orb612207) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human thyroid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (orb612207) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (orb612207) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (orb612207) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NRF1) Monoclonal Antibody, Unconjugated (orb612207) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Hela cell lysate, Primary: Anti-NRF1 (orb612207) at 1:1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 55 kD, Observed band size: 65 kD. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NRF1) monoclonal Antibody, Unconjugated (orb612207) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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