Phospho-IRF3 (Ser396) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6225
- Bilder (7)
| Artikelname: | Phospho-IRF3 (Ser396) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6225 |
| Hersteller Artikelnummer: | orb6225 |
| Alternativnummer: | BYT-ORB6225-100,BYT-ORB6225-200,BYT-ORB6225-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396 LHI(p-S)NS |
| Konjugation: | Unconjugated |
| Alternative Synonym: | IRF3 (p-S396), p-IRF3, phospho-IRF3, IIAE7, C920001K05Rik, IRF-3, IRF3_HUMAN, IRF3, IRF3_MOUSE, |
| Phospho-IRF3 (Ser396) Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-IRF3 (Ser396)) Polyclonal Antibody, Unconjugated (orb6225) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human myocardium), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-IRF3 (Ser396)) Polyclonal Antibody, Unconjugated (orb6225) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Heart tissue lysates, Lane 4: Rat Testis tissue lysates, Primary: Anti-Phospho-IRF3 (Ser396) (orb6225) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (orb6225) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (orb6225) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei. |
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Immunohistochemical analysis of formalin-fixed paraffin embedd |
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