KEAP1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6266
- Bilder (8)
| Artikelname: | KEAP1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6266 |
| Hersteller Artikelnummer: | orb6266 |
| Alternativnummer: | BYT-ORB6266-100,BYT-ORB6266-200,BYT-ORB6266-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human KEAP1 (65-160/624aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | INrf2, KLHL19, mKIAA0132, KEAP1_HUMAN, KEAP1, Cytosolic inhibitor of Nrf2 (INrf2), Kelch-like protein 19, KIAA0132, KEAP1_MOUSE, KEAP1_PIG, KEAP1_RAT, |
| KEAP1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 69 kDa |
| UniProt: | Q14145 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | KEAP1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
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Western blot analysis of rat kidney lysates(Lane1),rat brain lysates(Lane2) using KEAP1 antibody |
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HepG2 cells were incubated with Rabbit Anti-KEAP1 Polyclonal Antibody (orb6266) at 4C overnight and then mixed with Protein A agarose beads at 4C for 3hrs. The solutions were centrifuged and the pellets were washed with lysis buffer, heated, and subsequently analyzed by Western blotting. |
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Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (orb6266) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (orb6266) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (orb6266) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (orb6266) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (KEAP1) Polyclonal Antibody, Unconjugated (orb6266) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: A673 (Human) Cell Lysate at 30 ug, A431 (Human) Cell Lysate at 30 ug, Primary: Anti-KEAP1 (orb6266) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 69 kD, Observed band size: 69 kD. |
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