A recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.
Konjugation:
Unconjugated
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Application Notes: Optimal dilution of the recombinant FSP1 antibody should be determined by the researcher
IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.
IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).
Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481), Red=isotype control, Blue= FSP1 antibody.
Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.
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