Oct-4B/OCT4B-190 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6559
- Bilder (7)
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| Artikelname: | Oct-4B/OCT4B-190 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6559 |
| Hersteller Artikelnummer: | orb6559 |
| Alternativnummer: | BYT-ORB6559-50,BYT-ORB6559-100,BYT-ORB6559-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human OCT4 (201-300/360aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4, NF-A3, Oct-3/4, Otf-4, Otf3-rs7, Otf3g, PO5F1_HUMAN, POU5F1, Octamer-binding protein 3 (Oct-3), Octamer-binding protein 4 (Oct-4), Octamer-binding transcription factor 3 (OTF-3), PO5F1_MOUSE, |
| Oct-4B/OCT4B-190 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 21/39 kDa |
| UniProt: | Q01860 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | POU5F1 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green). |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (orb6559) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (orb6559) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (orb6559) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Oct-4B OCT4B-190) Polyclonal Antibody, Unconjugated (orb6559) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Heart (Mouse) Lysate at 40 ug, Lane 3: Cerebellum (Mouse) Lysate at 40 ug, Lane 4: Testis (Mouse) Lysate at 40 ug, Lane 5: Cerebrum (Rat) Lysate at 40 ug, Lane 6: Heart (Rat) Lysate at 40 ug, Lane 7: Cerebellum (Rat) Lysate at 40 ug, Primary: Anti-Oct-4B/OCT4B-190 (orb6559) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 47 kD. |
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IHC-P of rat brain tissue (dilution at:1:200) using Oct4B (OCT4B-190)NT antibody |
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