Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6569
- Bilder (8)
| Artikelname: | Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6569 |
| Hersteller Artikelnummer: | orb6569 |
| Alternativnummer: | BYT-ORB6569-50,BYT-ORB6569-100,BYT-ORB6569-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr57 TE(p-T)PL |
| Konjugation: | Unconjugated |
| Alternative Synonym: | CDKN1A (p-T57), p-CDKN1A, phospho-CDKN1A, CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1, CDKI, mda6, p21WAF, UV96, CDN1A_HUMAN, CDKN1A, CDK-interacting protein 1, Melanoma differentiation-associated protein 6 (MDA-6), PIC1, CDN1A_MOUSE, Melanoma differentiation-associated protein, |
| Phospho-CDKN1A/p21 (Thr57) Rabbit Polyclonal Antibody |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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IHC-P of human endometrial cancer using CDKN1A (phospho-Thr57) antibody. |
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Blank control: 293T. Primary Antibody (green line): Rabbit Anti-phospho-CDKN1A/p21 (Thr57) antibody (orb6569), dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-p21 (Thr57)) Polyclonal Antibody, Unconjugated (orb6569) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-CDKN1A/P21 (Thr57) Polyclonal Antibody, Unconjugated (orb6569) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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