Phospho-PKC delta (Thr505) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6750
- Bilder (7)
| Artikelname: | Phospho-PKC delta (Thr505) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6750 |
| Hersteller Artikelnummer: | orb6750 |
| Alternativnummer: | BYT-ORB6750-50,BYT-ORB6750-100,BYT-ORB6750-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Thr505 AS(p-T)FC |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PRKCD | PKC delta (p-T505), p-PKC delta, phospho-PKC delta, ALPS3, CVID9, MAY1, PKCD, nPKC-delta, D14Ertd420e, PKC[d], PKCdelta, KPCD_HUMAN, PRKCD, Tyrosine-protein kinase PRKCD, 2.7.11.13, KPCD_MOUSE, KPCD_RAT, |
| Phospho-PKC delta (Thr505) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 77 kDa |
| UniProt: | Q05655 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PRKCD |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-phospho-PKC delta (Thr505507) antibody (orb6750), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min) and then permeabilized with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6750) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6750) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6750) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-PKC delta (Thr505507)) Polyclonal Antibody, Unconjugated (orb6750) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat urinary bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-PKC delta (Thr505507)) Polyclonal Antibody, Unconjugated (orb6750) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Flow cytometric analysis of Mouse Splenocyte Cell using PKC delta (phospho-Thr505) antibody. |
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