SCD Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6919
- Bilder (7)
| Artikelname: | SCD Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6919 |
| Hersteller Artikelnummer: | orb6919 |
| Alternativnummer: | BYT-ORB6919-50,BYT-ORB6919-100,BYT-ORB6919-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SCD (265-359/359aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | FADS5, MSTP008, SCD1, SCDOS, hSCD1, Scd, Scd-1, ab, SCD_HUMAN, Acyl-CoA desaturase, Delta(9)-desaturase (Delta-9 desaturase), Fatty acid desaturase, 1.14.19.1, ACOD1_MOUSE, Delta(9)-desaturase 1 (Delta-9 desaturase 1), Fatty acid desaturase 1, Stearoyl-CoA desaturase 1, ACOD1_RAT, |
| SCD Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 41 kDa |
| UniProt: | O00767 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | SCD |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (SCD1) polyclonal Antibody, Unconjugated (orb6919) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (orb6919) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (orb6919) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (orb6919) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (orb6919) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated (orb6919) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated (orb6919) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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