Phospho-Smad2 (Ser465 + Ser467) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6979
- Bilder (6)
| Artikelname: | Phospho-Smad2 (Ser465 + Ser467) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6979 |
| Hersteller Artikelnummer: | orb6979 |
| Alternativnummer: | BYT-ORB6979-50,BYT-ORB6979-100,BYT-ORB6979-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467 CS(p-S)M(p-S) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | SMAD2 (p-S467), p-SMAD2, phospho-SMAD2, CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, 7120426M23Rik, Smad-2, mMad2, SMAD2_HUMAN, SMAD2, MAD homolog 2, Mothers against DPP homolog 2, Mad-related protein 2 (hMAD-2), SMAD family member 2 (SMAD 2 | Smad2 | hSMAD2), SMAD2_MOUSE, Mad-related protein 2 (mMad2), SMAD family member 2 (SMAD 2 | Smad2), SMAD2_RAT, Mad-related protein 2, |
| Phospho-Smad2 (Ser465 + Ser467) Rabbit Polyclonal Antibody |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-Phospho-Smad2 (Ser465 + Ser467) antibody (orb6979), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-Smad2 (Ser465 + Ser467)) polyclonal Antibody, Unconjugated (orb6979) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad2 (Ser465 + Ser467)) Polyclonal Antibody, Unconjugated (orb6979) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Smad2 (Ser465 + Ser467)) Polyclonal Antibody, Unconjugated (orb6979) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug, Lane 2: Raw264.7 (Mouse) Cell Lysate at 30 ug, Lane 3: Testis (Rat) Lysate at 40 ug, Lane 4: Hela (Human) Cell Lysate at 30 ug, Lane 5: HT1080 (Human) Cell Lysate at 30 ug, Lane 6: Jurkat (Human) Cell Lysate at 30 ug, Lane 7: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-Smad2 (Ser465 + Ser467) (orb6979) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD. |
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Western blot analysis of Mouse Muscle Lysate using Smad2 (phospho-Ser465-467) antibody |
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