A portion of amino acids 314-410 from the human protein was used as the immunogen for the CA9 antibody.
Konjugation:
Unconjugated
CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowmans capsule adjoining the outgoing proximal tubule. Reportedly, CAIX is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Application Verdünnung:
Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Anwendungsbeschreibung:
Application Notes: Optimal dilution of the CA9 antibody should be determined by the researcher
Western blot testing of human HEK293 cell lysate with CA9 antibody. Predicted molecular weight: 50-55 kDa.
Flow cytometry testing of FPA fixed human U-87 MG cells with CA9 antibody, Red=isotype control, Blue= CA9 antibody.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human kidney with CA9 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CA9 antibody. These results demonstrate the foremost specificity of the CA9/3406 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free CA9 antibody as confirmation of integrity and purity.
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