Cyclooxygenase 2 Recombinant Rabbit Monoclonal Antibody, Clone: [14D5], Unconjugated

Artikelnummer: BYT-ORB704294

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Artikelname:	Cyclooxygenase 2 Recombinant Rabbit Monoclonal Antibody, Clone: [14D5], Unconjugated
Artikelnummer:	BYT-ORB704294
Hersteller Artikelnummer:	orb704294
Alternativnummer:	BYT-ORB704294-50,BYT-ORB704294-100
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	A synthesized peptide derived from human COX2 (550-604/604aa)
Konjugation:	Unconjugated
Alternative Synonym:	COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2, PES-2, PHS II, Pghs2, TIS10, PGH2_HUMAN, PTGS2, Cyclooxygenase-2 (COX-2), Prostaglandin H2 synthase 2 (PGH synthase 2 \| PGHS-2), Prostaglandin-endoperoxide synthase 2, 1.14.99.1, PGH2_MOUSE, Glucocorticoid-regulated inflammatory cyclooxygenase, Macrophage activation-associated marker protein P71/73, TIS10 protein, Pghs-b, PGH2_RAT,

Cyclooxygenase 2 Recombinant Rabbit Monoclonal Antibody

Klonalität:	Recombinant
Konzentration:	1mg/ml
Klon-Bezeichnung:	[14D5]
Molekulargewicht:	65 kDa
UniProt:	P35354
Puffer:	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung:	Liquid
Target-Kategorie:	Ptgs2

Application Verdünnung:

WB=1:1000-2000, IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500

	A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (COX2/Cyclooxygenase 2) monoclonal Antibody, Unconjugated (orb704294) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
	Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (COX2/Cyclooxygenase 2) monoclonal Antibody, Unconjugated (orb704294) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
	Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cyclooxygenase 2) Monoclonal Antibody, Unconjugated (orb704294) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cyclooxygenase 2) Monoclonal Antibody, Unconjugated (orb704294) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
	Sample: Lane 1: A549 cell lysates, Primary: Anti-COX2/Cyclooxygenase 2 (orb704294) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.
	IHC-P analysis of human lung carcinoma using Cyclooxygenase 2 Monoclonal Antibody at 1:200 dilution.

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