Phospho-P38 MAPK (Thr180) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB7089
- Bilder (6)
| Artikelname: | Phospho-P38 MAPK (Thr180) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB7089 |
| Hersteller Artikelnummer: | orb7089 |
| Alternativnummer: | BYT-ORB7089-50,BYT-ORB7089-100,BYT-ORB7089-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated Synthesised phosphopeptide derived from human MAPK14 around the phosphorylation site of Thr180 EM(p-T)G |
| Konjugation: | Unconjugated |
| Alternative Synonym: | MAPK14 | p38 MAPK (p-T180/Y182), p-p38 MAPK, phospho-p38 MAPK, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, Crk1, p38-alpha, p38MAPK, p38a, Hog, p38Hog, MK14_CANLF, MAPK14, MAP kinase 14, MAPK 14, Mitogen-activated protein kinase p38 alpha (MAP kinase p38 alpha), 2.7.11.24, MK14_HUMAN, Cytokine suppressive anti-inflammatory drug-binding protein (CSAID-binding protein | CSBP), MAP kinase MXI2, MAX-interacting protein 2, Stress-activated protein kinase 2a (SAPK2a), MK14_MOUSE, MK14_RAT, |
| Phospho-P38 MAPK (Thr180) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 41 kDa |
| UniProt: | Q16539 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | MAPK14 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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Flow cytometric analysis of Hela cells using MAPK14 (phospho-Thr180) antibody. |
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Blank control (blue): Hela (fixed with 2% paraformaldehyde (10 min), then permeabilized with 0.3% tritionx-100 for 5 min at room temperature). Primary Antibody: Rabbit Anti-phospho-P38 MAPK (Thr180) antibody (orb7089) Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC), dilution: 1:200 in 1X PBS containing 0.5% BSA. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (p-P38 MAPK (Thr180)) Polyclonal Antibody, Unconjugated (orb7089) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
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Sample: Muscle (Mouse) Lysate at 40 ug, Muscle (Rat) Lysate at 40 ug, Primary: Anti-phospho-P38 MAPK (Thr180) (orb7089) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD. |
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