Phospho-PPAR Gamma (Ser112) Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB99282
- Bilder (8)
| Artikelname: | Phospho-PPAR Gamma (Ser112) Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB99282 |
| Hersteller Artikelnummer: | orb99282 |
| Alternativnummer: | BYT-ORB99282-50,BYT-ORB99282-100,BYT-ORB99282-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser112 PA(p-S)PP |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PPARG | PPAR gamma (p-S112), p-PPAR gamma, phospho-PPAR gamma, CIMT1, FPLD3, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma, PPAR-gamma, PPAR-gamma2, PPARgamma2, PPARG_HUMAN, PPARG, Nuclear receptor subfamily 1 group C member 3, PPARG_MOUSE, PPARG_RAT, |
| Phospho-PPAR Gamma (Ser112) Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 57 kDa |
| UniProt: | P37231 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PPARG |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg /Test, ELISA=1:5000-10000 |
| Anwendungsbeschreibung: | Modification: Phosphorylated |
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A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-PPAR Gamma (Ser112)) polyclonal Antibody, Unconjugated (orb99282) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Blank control (blue line): U251 (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-ho-PPAR Gamma (ser112) antibody (orb99282), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test. |
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phosphopeptide, non phosphopeptide. |
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Sample: Heart (Mouse) Lysate at 30 ug, Primary: Anti-Phospho-PPAR Gamma (ser112) (orb99282) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD. |
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Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: Large intestine (Mouse) Lysate at 40 ug, Lane 3: Large intestine (Rat) Lysate at 40 ug, Lane 4: Lung (Rat) Lysate at 40 ug, Primary: Anti-Phospho-PPAR Gamma (Ser112) (orb99282) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 51 kD, Observed band size: 51 kD. |
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Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PPAR Gamma (ser112) Polyclonal Antibody, Unconjugated (orb99282) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-PPAR Gamma (ser112) Polyclonal Antibody, Unconjugated (orb99282) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Western blot:Heart (Mouse),Primary:Anti-Phospho-PPAR Gamma (ser112) at 1:300 dilution. |
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