CDK1 Recombinant Monoclonal Antibody, Clone: [5C1], Unconjugated, Rabbit
Artikelnummer:
CSB-RA172307A0HU
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| Artikelname: | CDK1 Recombinant Monoclonal Antibody, Clone: [5C1], Unconjugated, Rabbit |
| Artikelnummer: | CSB-RA172307A0HU |
| Hersteller Artikelnummer: | CSB-RA172307A0HU |
| Alternativnummer: | CSB-RA172307A0HU-100UL, CSB-RA172307A0HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Cyclin-dependent kinase 1 (CDK1) (EC 2.7.11.22) (EC 2.7.11.23) (Cell division control protein 2 homolog) (Cell division protein kinase 1) (p34 protein kinase) , CDK1, CDC2 CDC28A CDKN1 P34CDC2 |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [5C1] |
| UniProt: | P06493 |
| Puffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Reinheit: | Affinity-chromatography |
| Formulierung: | Liquid |
| Target-Kategorie: | CDK1 |
| Antibody Type: | Recombinant Antibody |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
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Overlay Peak curve showing Hela cells stained with CSB-RA172307A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of A431 cell with CSB-RA172307A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) . |
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IHC image of CSB-RA172307A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA172307A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: K562 whole cell lysate(30µg) , Jurkat whole cell lysate(30µg) , Raji whole cell lysate(30µg) , HEK293 whole cell lysate(30µg) , MCF-7 whole cell lysate(30µg) , NTERA-2 whole cell lysate(30µg) All lanes: CDK1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time:2min |
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