SET Recombinant Monoclonal Antibody, Clone: [5E6], Unconjugated, Rabbit
Artikelnummer:
CSB-RA561645A0HU
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| Artikelname: | SET Recombinant Monoclonal Antibody, Clone: [5E6], Unconjugated, Rabbit |
| Artikelnummer: | CSB-RA561645A0HU |
| Hersteller Artikelnummer: | CSB-RA561645A0HU |
| Alternativnummer: | CSB-RA561645A0HU-100UL, CSB-RA561645A0HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, IHC, WB |
| Spezies Reaktivität: | Human |
| Konjugation: | Unconjugated |
| Alternative Synonym: | 2PP2A antibody, HLA DR associated protein II antibody, HLA-DR-associated protein II antibody, I 2PP2A antibody, I-2PP2A antibody, I2PP2A antibody, IGAAD antibody, Inhibitor of granzyme A activated DNase antibody, Inhibitor of granzyme A-activated DNase antibody, Inhibitor of GzmA-activated DNase antibody, inhibitor-2 of protein phosphatase-2A antibody, IPP2A2 antibody, PHAPII antibody, Phosphatase 2A inhibitor I2PP2A antibody, protein phosphatase type 2A inhibitor antibody, Protein SET antibody, Set antibody, SET nuclear oncogene antibody, SET translocation antibody, SET translocation (myeloid leukemia-associated) antibody, SET_HUMAN antibody, TAF I antibody, TAF IBETA antibody, TAF-I antibody, TAFI antibody, Template activating factor I antibody, Template-activating factor I antibody, Template-Activating Factor-I, chromatin remodelling factor antibody |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [5E6] |
| UniProt: | Q01105 |
| Puffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Reinheit: | Affinity-chromatography |
| Formulierung: | Liquid |
| Target-Kategorie: | SET |
| Antibody Type: | Recombinant Antibody |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
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Overlay Peak curve showing Hela cells stained with CSB-RA561645A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of Hela cell with CSB-RA561645A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) . |
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IHC image of CSB-RA561645A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA561645A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: HeLa whole cell lysate(30µg) , COLO205 whole cell lysate(30µg) , HEK293 whole cell lysate, HL-60 whole cell lysate(30µg) All lanes: SET antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 39 kDa Exposure time: 2min |
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