PKM Recombinant Monoclonal Antibody, Clone: [7B2], Unconjugated, Rabbit
Artikelnummer:
CSB-RA632595A0HU
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| Artikelname: | PKM Recombinant Monoclonal Antibody, Clone: [7B2], Unconjugated, Rabbit |
| Artikelnummer: | CSB-RA632595A0HU |
| Hersteller Artikelnummer: | CSB-RA632595A0HU |
| Alternativnummer: | CSB-RA632595A0HU-100UL, CSB-RA632595A0HU-50UL |
| Hersteller: | Cusabio |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, IHC, IP, WB |
| Spezies Reaktivität: | Human, Mouse |
| Konjugation: | Unconjugated |
| Alternative Synonym: | Pyruvate kinase PKM (EC 2.7.1.40) (Cytosolic thyroid hormone-binding protein) (CTHBP) (Opa-interacting protein 3) (OIP-3) (Pyruvate kinase 2/3) (Pyruvate kinase muscle isozyme) (Thyroid hormone-binding protein 1) (THBP1) (Tumor M2-PK) (p58) , PKM, OIP3 PK2 PK3 PKM2 |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [7B2] |
| UniProt: | P14618 |
| Puffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Reinheit: | Affinity-chromatography |
| Formulierung: | Liquid |
| Target-Kategorie: | PKM |
| Antibody Type: | Recombinant Antibody |
| Application Verdünnung: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, FC:1:20-1:200, IP:1:200-1:1000 |
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Overlay histogram showing HepG2 cells stained with CSB-RA632595A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*106 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
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Immunofluorescence staining of Hela Cells with CSB-RA632595A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) . |
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IHC image of CSB-RA632595A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
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Immunoprecipitating PKM in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA632595A0HU in Hela whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA632595A0HU(2µg) + Hela whole cell lysate(500µg) Lane 3: Hela whole cell lysate (10µg) |
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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue All lanes: PKM antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 59, 57 kDa Observed band size: 58 kDa |
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