The antiserum was produced against synthesized peptide derived from the Internal region of human LMNB1. AA range:391-440
Alternative Synonym:
LMNB1, LMN2, LMNB, Lamin-B1
lamin B1(LMNB1) Homo sapiens This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015],
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA (F:GCUUCUUGAUGUAAAGUUATT,R:UAACUUUACAUCAAGAAGCTT), (Green) primary antibody was diluted at 1:1000, 4over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37 1hour. (Red) actin Monoclonal Antibody (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4 over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37 1hour.
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