The antiserum was produced against synthesized peptide derived from human ZP1. AA range:221-270
Alternative Synonym:
ZP1, Zona pellucida sperm-binding protein 1, Zona pellucida glycoprotein 1, Zp-1
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene ensures the structural integrity of the zona pellucida. Mutations in this gene are a cause of oocyte maturation defect and infertility. [provided by RefSeq, May 2014],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Western Blot analysis of LOVO cells using ZP1 Polyclonal Antibody. Secondary antibody(catalog:RS0002) was diluted at 1:20000
Western blot analysis of lysates from LOVO cells, using ZP1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HeLa cells using ZP1 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4 overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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