The antiserum was produced against synthesized peptide derived from human FKHR around the phosphorylation site of Ser256. AA range:223-272
Alternative Synonym:
FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunofluorescence analysis of Hela cell. 1,FoxO1 (phospho Ser256) Polyclonal Antibody(red) was diluted at 1:200(4 overnight). Active Caspase-3 Monoclonal Antibody(5E1)(green) was diluted at 1:200(4 overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
Western Blot analysis of HELA cells using Phospho-FoxO1 (S256) Polyclonal Antibody diluted at 1:1000
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FKHR (Phospho-Ser256) Antibody
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using FKHR (Phospho-Ser256) Antibody. The picture on the right is blocked with the phospho peptide.
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