CYP27A1 (5H15) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09642
Produktübersicht
Artikelname:
CYP27A1 (5H15) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09642
Hersteller Artikelnummer:
AMRe09642
Alternativnummer:
EKL-AMRE09642-50UL, EKL-AMRE09642-100UL, EKL-AMRE09642-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, IHC, WB
Spezies Reaktivität:
Human, Mouse, Rat
Konjugation:
Unconjugated
Alternative Synonym:
CP27, CTX, CYP, CYP27, CYP27A1,
Produktbeschreibung
Catalyzes the first step in the oxidation of the side chain of sterol intermediates, the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (PubMed:9660774, PubMed: 12077124, PubMed: 21411718, PubMed: 28190002). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the neutral (classic) and the acid (alternative) pathways (PubMed: 9660774, PubMed: 1708392, PubMed: 11412116, PubMed: 2019602, PubMed: 7915755, PubMed: 9186905, PubMed: 9790667). May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (PubMed: 9660774, PubMed: 12077124). Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation (PubMed: 28190002). Also hydroxylates retinal 7- ketocholesterol, a noxious oxysterol with pro-inflammatory and pro- apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium (PubMed: 21411718). May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PubMed: 15465040).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
60kDa
NCBI:
1593
UniProt:
Q02318
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
CYP27A1
Anwendungsbeschreibung
Application Verdünnung:
WB 1:1000-1:5000,IHC 1:100-1:200,ICC/IF 1:50-1:100,FC 1:50-1:200
