Cytochrome P450 1A2 (8B7) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09712
Produktübersicht
Artikelname:
Cytochrome P450 1A2 (8B7) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09712
Hersteller Artikelnummer:
AMRe09712
Alternativnummer:
EKL-AMRE09712-50UL, EKL-AMRE09712-100UL, EKL-AMRE09712-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
FC, ICC, WB
Spezies Reaktivität:
Human
Konjugation:
Unconjugated
Alternative Synonym:
CP12, CYP1A2, CYPIA2, P3 450, P450 4, P450 P3,
Produktbeschreibung
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 11555828, PubMed: 12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta- estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed: 11555828, PubMed: 12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed: 21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed: 19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed: 9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH- independent) (PubMed: 21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed: 14725854). Metabolizes caffeine via N3-demethylation (Probable).
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
58kDa
NCBI:
1544
UniProt:
P05177
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
CYP1A2
Anwendungsbeschreibung
Application Verdünnung:
WB 1:1000-1:5000,ICC/IF 1:200-1:500,FC 1:50-1:200
