Cytochrome P450 3A4 (12D7) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09714
Produktübersicht
Artikelname:
Cytochrome P450 3A4 (12D7) Rabbit Monoclonal Antibody, Unconjugated
Artikelnummer:
EKL-AMRE09714
Hersteller Artikelnummer:
AMRe09714
Alternativnummer:
EKL-AMRE09714-50UL, EKL-AMRE09714-100UL, EKL-AMRE09714-200UL
Hersteller:
EnkiLife
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IF, IHC, IP, WB
Spezies Reaktivität:
Human
Konjugation:
Unconjugated
Alternative Synonym:
CYP3A4, CYPIIIA3, CYPIIIA4, Cytochrome P450 3A3, Cytochrome P450 HLp, Cytochrome P450 NF-25, Cytochrome P450-PCN1, Nifedipine oxidase,
Produktbeschreibung
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide. A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids (PubMed:10681376, PubMed: 11093772, PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15373842, PubMed: 15764715, PubMed: 20702771, PubMed: 19965576, PubMed: 21490593, PubMed: 21576599). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 2732228, PubMed: 14559847, PubMed: 12865317, PubMed: 15373842, PubMed: 15764715, PubMed: 21576599, PubMed: 21490593). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta- estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317). Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone (PubMed: 2732228, PubMed: 15373842, PubMed: 15764715, PubMed: 22773874). Metabolizes testosterone to less biologically active 2beta- and 6beta- hydroxytestosterones (PubMed: 2732228, PubMed: 15373842, PubMed: 15764715). Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C- 4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosyn
Produkteigenschaften
Klonalität:
Monoclonal
Molekulargewicht:
57kDa
NCBI:
1576
UniProt:
P08684
Puffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4C short term. Store at -20C long term. Avoid freeze / thaw cycle.
Reinheit:
Affinity purification
Formulierung:
Liquid
Target-Kategorie:
CYP3A4
Anwendungsbeschreibung
Application Verdünnung:
WB 1:500-1:2000,IHC 1:100-1:200,IP 1:10-1:100,IF-P 1:100-1:200
