Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Formulierung:
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Application Verdünnung:
IF (10 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 1 µg/ml)
Anwendungsbeschreibung:
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Preliminary experiments Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 gave no specific signal but low background (at antibody concentration up to 1 ug/ml).
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