Elastin antibody LS-C744107 is an unconjugated rabbit polyclonal antibody to Elastin (ELN) from rat. It is reactive with mouse and rat. Validated for ELISA, IF, IHC and RIA.
After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solutio
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