The MD Cryo-EM Vitrification Screen is a rationally designed additive screen of 19 different conditions aimed at improving vitrification outcomes in single-particle cryo-electron microscopy workflows. It systematically addresses the most common sample preparation bottlenecks, including air-water interface (AWI) adsorption, preferred orientation, aggregation, and uneven ice - by providing a diverse set of validated detergents and surfactants for buffer and additive screening.
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