This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
SDS-PAGE Analysis of Purified CDH16 Rabbit Recombinant Monoclonal Antibody (CDH16/7028R). Confirmation of Purity and Integrity of Antibody.
IHC analysis of formalin-fixed, paraffin-embedded human thyroid. Stained using CHD16/7028R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.
IHC analysis of formalin-fixed, paraffin-embedded human renal cell carcinoma. Stained using CDH16/7028Rat 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.
Western Blot Analysis of Kidney tissue lysate of different species using Ksp-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH16/7028R).
Western Blot Analysis of human kidney tissue lysate using Ksp-Cadherin Recombinant Rabbit Monoclonal Antibody (CDH16/7028R).
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