Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. Its epitope spans between aa 83-204 of p27. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Flow Cytometry of human p27 on HeLa cells. Blac
Formalin-fixed, paraffin-embedded human Cervical Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)
Formalin-fixed, paraffin-embedded human Colon Cancer stained with p27 Monoclonal Antibody (DCS-72.F6)
Flow Cytometry of human p27 on HeLa cells. Black: cells alone, Grey: Isotype Control, Green: AF488-labeled p27 Monoclonal Antibody (DCS-72.F6).
SDS-PAGE Analysis of Purified p27 MAb (DCS-72.F6). Confirmation of Purity and Integrity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
