Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)
Alternative Synonym:
Deoxynucleotidyltransferase terminal, DNA Nucleotidylexotransferase, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase, Terminal transferase
Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3-hydroxyl end of DNA primers without template direction. The enzyme thus provides a unique method for the labeling of the 3 termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 9
Formalin-fixed, paraffin-embedded human Thymus sta
Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
Formalin-fixed, paraffin-embedded human Thymus stained with TdTMouse Monoclonal Antibody (DNTT/1453).
SDS-PAGE Analysis of Purified TdT Mouse Monoclonal Antibody (DNTT/1453). Confirmation of Purity and Integrity of Antibody.
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