Recombinant fragment of human AKT1 protein (around aa 85-189) (exact sequence is proprietary)
Alternative Synonym:
Oncogene AKT1, PKB, PKB-ALPHA, PRKBA, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC Alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Recognizes a protein of 62kDa, which is identified as AKT1The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBA°a or RacPK-A°a) and Akt 3 (also designated PKBA or thymoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-A°a tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Formalin-fixed, paraffin-embedded human colon carcinoma stained with AKT1 Recombinant Mouse Monoclonal Antibody (rAKT1/2491).
SDS-PAGE Analysis of Purified AKT1 Recombinant Mouse Monoclonal Antibody (rAKT1/2491). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of MCF-7 cell lysate using AKT1 Recombinant Mouse Monoclonal Antibody (rAKT1/2491).
Western blot analysis of Human Brain, Human Kidney, Human Liver, Human Lung, Human Pancreas, Human Sk. Muscle and Human Spleen tissue lysates using AKT1 Recombinant Mouse Monoclonal Antibody (rAKT1/2491).
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