Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)
Alternative Synonym:
DKFZp761K2222, TIP27, ZNF802
JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunoprecipitation (1-2ug per 100-500ug of total protein),Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed ti
SDS-PAGE Analysis Purified JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence analysis of PFA-fixed HeLa cells. JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow cytometric analysis of PFA-fixed HeLa cells. JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Formalin-fixed, paraffin-embedded human testis stained. Strong nuclear staining using JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
Analysis of Protein Array containing more than 19,000 full-length human proteins using JAZF1 Mouse Monoclonal Antibody (PCRP-JAZF1-1C2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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