GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 2
Formalin-fixed, paraffin-embedded human urothelia
Formalin-fixed, paraffin-embedded human lobular breast carcinoma stained with GATA-3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human urothelial carcinoma stained with GATA-3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870).
Western blot analysis of MCF-7 cell lysate using GATA-3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870). Specific bands were detected for GATA-3 full length (FL) at approx. 50 kDa and the splice form (SF) at approximately 37 kDa (as indicated).
Western blot analysis of Jurkat cell lysate using GATA3 Recombinant Mouse Monoclonal Antibody (rGATA3/3870).
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