Bovine, Gallus, Human, Mouse, Porcine, Rabbit, Rat
Immunogen:
GFAP isolated from pig spinal cord (GA-5), Recombinant GFAP protein (ASTRO/789)
Alternative Synonym:
Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with
Formalin-fixed, paraffin-embedded Rat
Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).
Formalin-fixed, paraffin-embedded human Schwanoma stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with GFAP Monoclonal Antibody (GA-5 + ASTRO/789).
Western Blot Analysis of human brain tissue lysate using GFAP MAb (GA-5 + ASTRO/789).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
