Besides its role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Immunofluorescence (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed b
SDS-PAGE Analysis Purified GPI Mouse Monoclonal Antibody (CPTC-GPI-1). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of human MCF-7 cells labeling GPI with GPI Mouse Monoclonal Antibody (CPTC-GPI-1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red)
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with GPI Mouse Monoclonal Antibody (CPTC-GPI-1).
Western Blot Analysis of T47D, PC3, HePG2 cell lysates using GPI Mouse Monoclonal Antibody (CPTC-GPI-1).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Glucose 6-Phosphate Isomerase Monoclonal Antibody (CPTC-GPI-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of Human Kidney tissue lysate using GPI Mouse Monoclonal Antibody (CPTC-GPI-1).
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