Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
200ug/ml of Ab Purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).
SDS-PAGE Analysis Purified Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R). Confirmation of Integrity and Purity of Antibody.
Flow Cytometric Analysis of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (Blue), Isotype Control (Red).
Immunofluorescent staining of PFA-fixed HeLa cells using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R) followed by goat anti-rabbit IgG-CF488 (green). Membrane labeled with Phalloidin.
Western Blot Analysis of human heart tissue lysate using Histone H1 Rabbit Recombinant Monoclonal Antibody (HH1/1784R).
Western Blot Analysis of Human Spleen, A431, and HeLa lysates using Histone H1 Recombinant Rabbit Monoclonal Antibody (HH1/1784R).
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