Activated human peripheral blood mononuclear cells
Alternative Synonym:
DR alpha chain, DRB1, DRB4, HLA class II histocompatibility antigen, HLA class II histocompatibility antigen DR alpha chain, HLA DR1B, HLA DR3B, HLA DRA, HLA DRA1, HLA DRB1, HLA DRB3, HLA DRB4, HLA DRB5, HLA-DRA, HLADR4B, HLADRA1, HLADRB, Major histocompatibility complex class II DR alpha, Major histocompatibility complex class II DR beta 1, Major histocompatibility complex class II DR beta 3, Major histocompatibility complex class II DR beta 4, Major histocompatibility complex class II DR beta 5, MHC cell surface glycoprotein, MHC class II antigen DRA
This MAb reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (2-4ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DR Monoclonal Antibody (SPM289).
Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DR Monoclonal Antibody (SPM289).
Western Blot Analysis of Ramos cells and human spleen tissue lysate using HLA-DR Monoclonal Antibody (SPM289).
Immunofluorescence Analysis of Raji cells labeling HLA-DR with HLA-DR Monoclonal Antibody (SPM289) conjugated with APC (Green) The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of human Raji cells using HLA-DR Monoclonal Antibody (SPM289) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
SDS-PAGE Analysis of Purified HLA-DRB Mouse Monoclonal Antibody (SPM289). Confirmation of Purity and Integrity of Antibody.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
