DRB1, HLA class II histocompatibility antigen, DR-1 beta chain, HLA-DR-beta 1, HLA-DRB1, human leucocyte antigen DRB1, Leucocyte antigen DR beta 1 chain, lymphocyte antigen DRB1, major histocompatibility complex, class II, DR beta 1, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR-beta cell surface glycoprotein
This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. vC OOIt does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml),,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20
Western blot analysis of HDLM-2 cell line lysate using HLA-DRB Rabbit Recombinant Monoclonal Antibody (HLA-DRB/7795R).
Western blot analysis of Raji cell line lysate using HLA-DRB Rabbit Recombinant Monoclonal Antibody (HLA-DRB/7795R).
Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DR Monoclonal Antibody (HLA-DRB/7795R). Inset: PBS instead of primary antibody, secondary only negative control.
SDS-PAGE Analysis of Purified HLA-DR Monoclonal Antibody (HLA-DRB/7795R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human lymph node stained with HLA-DR Monoclonal Antibody (HLA-DRB/7795R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of HDLM-2 cell lysate using HLA-DR Monoclonal Antibody (HLA-DRB/7795R).
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