Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)
HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian (TM)-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for
SDS-PAGE Analysis of Purified HSP27 Recombinant Rabbit Monoclonal Antibody (HSPB1/6490R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human thyroid stained with HSP27 Recombinant Rabbit Monoclonal Antibody (HSPB1/6490R).
Western blot analysis of human liver tissue lysate using HSP27 Recombinant Rabbit Monoclonal Antibody (HSPB1/6490R).
Western blot analysis of Heart, Skeletal muscle, and Skin tissue lysates of different species using HSP27 Recombinant Rabbit Monoclonal Antibody (HSPB1/6490R).
Western blot analysis of Human Heart, Human Liver, Human Skeletal muscle, Human Skin, A549 and HeLa lysates using HSP27 Recombinant Rabbit Monoclonal Antibody (HSPB1/6490R).
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