Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-(TM)and A°a) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-(TM)and interferon-A°agenes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth, overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3Ap48 and IFN consensus sequence-binding protein (ICSBP).
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),,Optimal dilution for a specific application should be determined.
Western blot analysis of MCF-7 cell lysate using IRF3 Mouse Monoclonal Antibody (PC
Immunofluorescence Analysis of HeLa cells using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E11) followed by goat anti-mouse IgG-CF488 (green). Counterstain is phalloidin.
Western blot analysis of MCF-7 cell lysate using IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E11).
SDS-PAGE Analysis of Purified IRF3 Mouse Monoclonal Antibody (PCRP-IRF3-1E11). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteinsusing IRF-3Monospecific Mouse Monoclonal Antibody (PCRP-IRF3-1E11). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
