This MAb recognizes a protein of 56kDa, identified as cytokeratin 6A (KRT6A). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns. Cytokeratin 6A is the dominant form in epithelial tissue. Cytokeratin 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Cytokeratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express KRT6 and KRT16. KRT6 is strongly expressed in about 75% of head and neck squamous cell carcinomas..
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooli
Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with Cytokeratin 6A (KRT6A) Mouse Monoclonal Antibody (KRT6A/2368).
Formalin-fixed, paraffin-embedded human basal cell carcinomastained with Cytokeratin 6A (KRT6A) Mouse Monoclonal Antibody (KRT6A/2368).
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 6A (KRT6A) Mouse Monoclonal Antibody (KRT6A/2368).
SDS-PAGE Analysis Purified Cytokeratin 6A Mouse Monoclonal Antibody (KRT6A/2368). Confirmation of Integrity and Purity of Antibody.
Flow cytometric analysis of PFA-fixed HeLacells. KRT6AMouse Monoclonal Antibody (KRT6A/2368) followed bygoat anti-mouse IgG-CF488 (blue), isotype control (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 6A(KRT6A) Mouse Monoclonal Antibody (KRT6A/2368). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD&apos,s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD&apos,s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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