This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1m
Formalin-fixed, paraffin-embedded rat colon stained with Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).
Western blot analysis of human A) stomach B) intestine tissue lysatesusing Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).
Confocal immunofluorescence analysis of MeOH-fixed HeLa cells. Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190) followed by goat anti-mouse IgG-CF488 (green). Nuclei counterstained with RedDot.
Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).
Western Blot Analysis of human A549 and HEP-2 cell lysates using Cytokeratin 18 Mouse Monoclonal Antibody (KRT18/1190).
SDS-PAGE Analysis of Purified Keratin 18 Recombinant Rabbit Monoclonal Antibody (KRT18/1190). Confirmation of Purity and Integrity of Antibody.
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