This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells),Western Blot (1-2ug/ml),Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA,
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).
SDS-PAGE Analysis of Purified Cytokeratin 18 Rabbit Recombinant Monoclonal Antibody (KRT18/2819R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).
Immunofluorescence Analysis of MCF-7 cells labeling KRT18 with Cytokeratin 18 Rabbit Recombinant MAb (KRT18/2819R followed by Goat anti-Rabbit IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Rabbit Recombinant MAb(KRT18/2819R) followed by Goat anti-rabbit IgG-CF488 (Blue), Isotype Control (Red).
Western Blot Analysis of Human Intestine, Human Liver, Human Lung, A431, and HeLa lysates using Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody (KRT18/2819R).
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