EpCAM / CD326 (Epithelial Marker) Antibody, IgG1, Clone: [323/A3], Mouse, Monoclonal

Artikelnummer: NBT-4072-MSM3-P1ABX
Artikelname: EpCAM / CD326 (Epithelial Marker) Antibody, IgG1, Clone: [323/A3], Mouse, Monoclonal
Artikelnummer: NBT-4072-MSM3-P1ABX
Hersteller Artikelnummer: 4072-MSM3-P1ABX
Alternativnummer: NBT-4072-MSM3-P1ABX-100
Hersteller: NeoBiotechnologies
Wirt: Mouse
Kategorie: Antikörper
Applikation: FC, IHC
Spezies Reaktivität: Human
Immunogen: MCF-7 human breast cancer cells
Alternative Synonym: Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, Membrane Component, Chromosome 17, Surface Marker-1 (M17S1), REG-1, Reggie-1, Reggie-2
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Klonalität: Monoclonal
Klon-Bezeichnung: [323/A3]
Molekulargewicht: 40-43kDa
Isotyp: IgG1
NCBI: 4072
UniProt: P16422
Formulierung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type: Monoclonal Antibody
Anwendungsbeschreibung: Flow Cytometry (1-2ug/million cells), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooli
Formalin-fixed, paraffin-embedded human colon stained with EpCAM / C
Formalin-fixed, paraffin-embedded human colon carcinoma stained with EpCAM / CD326 Mouse Monoclonal Antibody (323/A3). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human colon stained with EpCAM / CD326 Mouse Monoclonal Antibody (323/A3). Inset: PBS instead of primary antibody, secondary only negative control.
Flow cytometric analysis of PFA-fixed MCF-7 cells. EpCAM / CD326 Mouse Monoclonal Antibody (323/A3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
SDS-PAGE Analysis of Purified EpCAM / CD326 Mouse Monoclonal Antibody (323/A3). Confirmation of Integrity and Purity of Antibody.
SDS-PAGE Analysis Purified EpCAM Mouse Monoclonal Antibody (323/A3). Confirmation of Purity and Integrity of Antibody.