Epitope of this MAb is mapped between aa 202-212 of EGP40, which is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Antibody to Ep-CAM has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
200ug/ml of Ab purified by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Anwendungsbeschreibung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
SDS-PAGE Analysis of Purified EpCAM Recombinant Rabbit Antibody (EGP40/8191R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of (1) human lung, (2) mouse lung, (3) rat lung, (4) hamster lung, (5) guinea pig lung, (6) human intestine, (7) mouse intestine, (8) rat intestine, (9) hamster intestine and (10) guinea pig intestine tissue lysates using EpCAM Recombinant Rabbit Monoclonal Antibody (EGP40/8191R).
IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Negative tissue control using EGP40/8191R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.
