hMAD-3, hSMAD3, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MADH3, SMA and MAD related protein 3, SMAD, mothers against DPP homolog 3
Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been implicated as downstream effectors of TGFA°a/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP-2 and BMP-4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFA°a and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFA°a signaling by interfering with TGFA°a-mediated phosphorylation of other Smad family members.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Optimal dilution for a specific application should be determined.
Immunofluorescence Analysis of PFA-fixed HeLa cells using SMAD3 Mouse Monoclonal Antib
Flow Cytometric Analysis of PFA-fixed HeLa cells. SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
Immunofluorescence Analysis of PFA-fixed HeLa cells using SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE Analysis. Purified SMAD3 Mouse Monoclonal Antibody (PCRP-SMAD3-1A2). Confirmation of Purity and Integrity of Antibody.
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