Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody, Clone: [MGMT/8319R], Rabbit, Monoclonal
Artikelnummer:
NBT-4255-RBM10-P0
Hersteller Artikelnummer:
4255-RBM10-P0
Alternativnummer:
NBT-4255-RBM10-P0-20
Hersteller:
NeoBiotechnologies
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Purified His-tagged MGMT protein
Alternative Synonym:
6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross OOlinks at the O6 OO alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 OOposition of guanine in DNA (prior to cross OOlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA OOdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Western Blot Analysis of MCF-7 lysate using MGMT Rabbit Recombinant Monoclonal Antibody (MGMT/8319R).
Western Blot Analysis of MCF-7, LNCaP, Hep G2 and human liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8319R).
Western blot analysis of Human Liver, Mouse Liver, Rat Liver, Hamster Liver and Guinea pig Liver lysates using MGMT Recombinant Rabbit Monoclonal Antibody (MGMT/8319R).
Western Blot Analysis of MCF-7 lysate using MGMT Rabbit Recombinant Monoclonal Antibody (MGMT/8319R).
SDS-PAGE Analysis of Purified MGMT Mouse Monoclonal Antibody (MGMT/8319R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with MGMT Mouse Monoclonal Antibody (MGMT/8319R). Inset: PBS instead of primary antibody, secondary only negative control.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
